This biochemical assay determines the flexibility of a microorganism to supply the enzyme lysine decarboxylase. The enzyme removes the carboxyl group from the amino acid lysine, producing cadaverine and carbon dioxide. A optimistic result’s sometimes indicated by a coloration change within the progress medium from yellow to purple, reflecting an alkaline pH shift brought on by the buildup of cadaverine. A detrimental result’s indicated by a yellow coloration, that means no decarboxylation has occurred.
Differentiating bacterial species is essential for correct identification in scientific, analysis, and industrial settings. This particular assay supplies priceless data for distinguishing enterobacteria and different gram-negative bacilli, significantly in situations the place phenotypic traits overlap. Its historic significance lies in providing a comparatively easy and cost-effective methodology for microbial classification, contributing to a deeper understanding of bacterial metabolism and variety.
